Cloning and characterization of cuticle-degrading serine protease from nematode-trapping fungus Arthrobotrys musiformis
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چکیده
منابع مشابه
Purification and cloning of an extracellular serine protease from the nematode-trapping fungus Monacrosporium cystosporium.
An extracellular protease (Mc1) was isolated from the nematode-trapping fungus Monacrosporium cystosporium by gel filtration, anion-exchange, and hydrophobic interaction chromatographies. This protease had a molecular mass of approximately 38 kDa and displayed an optimal activity at pH 7-9 and 56 degrees (over 30 min). Its proteolytic activity was highly sensitive to the serine protease inhibit...
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Over one-year period, 150 pasture soil samples and 138 sheep faecal samples, collected from different parts of Iran were screened for the presence of nematophagous fungi. The samples were cultured at 25ºC on chloramphenicol-2% water agar (CHF-WA) plates in the presence of Haemonchus contortus third stage larvae (L3) and checked over a two-month period for characteristic conidia, conidiophores a...
متن کاملMolecular characterization of an expressed sequence tag representing the cuticle-degrading serine protease gene (PII) from the nematophagous fungus Arthrobotrys oviformis by differential display technology.
The technology of mRNA-based differential display reverse transcriptase-polymerase chain reaction (DDRT-PCR) was used to detect a 246-bp differentially expressed fragment from the nematophagous fungus Arthrobotrys oviformis when young mycelia were induced with the round worm Haemonchus contortus. The fragment was converted into an expressed sequence tag (EST) through characterization at the mol...
متن کاملIsoenzyme analysis of Arthrobotrys, a nematode-trapping fungus.
Extraction and isoenzyme analysis of four isolates of Arthrobotrys including A. musiformis, A. robusta and A. conoides were conducted. Among the 14 enzymes studied by starch gel electrophoresis, using morpholine-citrate as gel/electrode buffer, the following nine enzymes showed interpretable banding patterns: alpha-esterase, fumarase, hexokinase, isocitrate dehydrogenase, leucine aminopeptidase...
متن کاملSurface polymers of the nematode-trapping fungus Arthrobotrys oligospora.
The nematophagous fungus Arthrobotrys oligospora captures nematodes using adhesive polymers present on special hyphae (traps) which form a three-dimensional network. To understand further the adhesion mechanisms, A. oligospora surface polymers were visualized by transmission electron microscopy and characterized by chemical methods. Both traps and hyphae were surrounded by a fibrillar layer of ...
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ژورنال
عنوان ژورنال: Mycoscience
سال: 2016
ISSN: 1340-3540
DOI: 10.1016/j.myc.2015.12.003